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rna bee  (AMS Biotechnology)


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    AMS Biotechnology rna bee
    Rna Bee, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+bee/RNA-Bee%2C+discontinued%3A+see+replacement+RNA+STAT-60/bio_rxiv__64898__2026__03__01__708896-207-9-10
    Average 96 stars, based on 23 article reviews
    rna bee - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Sonication:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Protein Extraction:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Western Blot:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    RNA Extraction:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Amyloid pathology reduces ELP3 expression and tRNA modifications leading to impaired proteostasis.
    Article Snippet: For qPCR and Western blot analysis, tissue was homogenized in buffer containing 1 % NP-40, 0.5 % sodium deoxycholate, 0.1 % SDS and protease inhibitor cocktail (Sigma Aldrich, #P8340). .. For RNA extraction, tissue homogenate was mixed in an equal volume of RNA-Bee (Amsbio, CS-104B) and RNA was isolated using phenol-chloroform extraction and a Purelink RNA Mini Kit (Life Technologies, 12183020) with an on-column DNAse Purelink Kit (Life Technologies, 12183025). .. 1000–500 ng of RNA were converted to cDNA with the High-Capacity RNA-to-cDNA kit (Applied biosystems, 4388950) and qPCR was performed on StepOne Real Time PCR System with Taqman Assays (Life Technologies).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic Targeting of Microglial Hexokinase-2 Recalibrates Inflammasome Activation and Improves Functional Recovery After Traumatic Brain Injury
    Article Snippet: .. The remaining part was stored at −80 °C in RNA-Bee (Amsbio, CS-501B) for later RNA extraction and gene expression analysis. ..

    Gene Expression:

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at −80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism in Alzheimer’s disease reveals a critical reliance on Hexokinase 2 dosage on microglial activation and disease progression
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from subjects with AD were homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.
    Article Snippet: .. One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis, and the other was stored at -80 C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis. .. Post-mortem brain tissues from patients with ADwere homogenized in RIPA buffer with a protease inhibitor cocktail (Roche) and a dilution of brain to RIPA of 1:10 (w/v).

    Article Title: Therapeutic Targeting of Microglial Hexokinase-2 Recalibrates Inflammasome Activation and Improves Functional Recovery After Traumatic Brain Injury
    Article Snippet: .. The remaining part was stored at −80 °C in RNA-Bee (Amsbio, CS-501B) for later RNA extraction and gene expression analysis. ..

    Isolation:

    Article Title: Amyloid pathology reduces ELP3 expression and tRNA modifications leading to impaired proteostasis.
    Article Snippet: For qPCR and Western blot analysis, tissue was homogenized in buffer containing 1 % NP-40, 0.5 % sodium deoxycholate, 0.1 % SDS and protease inhibitor cocktail (Sigma Aldrich, #P8340). .. For RNA extraction, tissue homogenate was mixed in an equal volume of RNA-Bee (Amsbio, CS-104B) and RNA was isolated using phenol-chloroform extraction and a Purelink RNA Mini Kit (Life Technologies, 12183020) with an on-column DNAse Purelink Kit (Life Technologies, 12183025). .. 1000–500 ng of RNA were converted to cDNA with the High-Capacity RNA-to-cDNA kit (Applied biosystems, 4388950) and qPCR was performed on StepOne Real Time PCR System with Taqman Assays (Life Technologies).

    Extraction:

    Article Title: Amyloid pathology reduces ELP3 expression and tRNA modifications leading to impaired proteostasis.
    Article Snippet: For qPCR and Western blot analysis, tissue was homogenized in buffer containing 1 % NP-40, 0.5 % sodium deoxycholate, 0.1 % SDS and protease inhibitor cocktail (Sigma Aldrich, #P8340). .. For RNA extraction, tissue homogenate was mixed in an equal volume of RNA-Bee (Amsbio, CS-104B) and RNA was isolated using phenol-chloroform extraction and a Purelink RNA Mini Kit (Life Technologies, 12183020) with an on-column DNAse Purelink Kit (Life Technologies, 12183025). .. 1000–500 ng of RNA were converted to cDNA with the High-Capacity RNA-to-cDNA kit (Applied biosystems, 4388950) and qPCR was performed on StepOne Real Time PCR System with Taqman Assays (Life Technologies).



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